This is an R Markdown website, created by Julia Wrobel for a single-cell spatial proteomics and multiplex imaging data workshop at the 2024 Pacific Symposium on Biocomputing (PSB). For raw materials, go to the GitHub repository for this website.

In the short course, we will use several packages which are listed below. Code to install the packages follows, and version information about each of these packages and their dependencies is at the bottom of this page.

Installing packages from CRAN

# main packages
install.packages(c("tidyverse", "spatstat", "spatialTIME", "mxnorm","survival", "scSpatialSim"))

# additional packages used for visualization
install.packages(c("viridis", "patchwork"))

Installing packages from GitHub

# install devtools
install.packages("devtools")

# mxfda
devtools::install_github("julia-wrobel/mxfda")

Installing packages from Bioconductor

# install Bioconductor
BiocManager::install(version = "3.16")

# VectraPolarisData
BiocManager::install("VectraPolarisData")

Versions of key packages:

devtools::session_info(c("tidyverse", "VectraPolarisData", "spatstat", "spatialTIME", "mxfda"))
## ─ Session info ───────────────────────────────────────────────────────────────
##  setting  value
##  version  R version 4.3.1 (2023-06-16)
##  os       macOS Sonoma 14.1.1
##  system   aarch64, darwin20
##  ui       X11
##  language (EN)
##  collate  en_US.UTF-8
##  ctype    en_US.UTF-8
##  tz       Europe/Berlin
##  date     2023-12-20
##  pandoc   3.1.1 @ /Applications/RStudio.app/Contents/Resources/app/quarto/bin/tools/ (via rmarkdown)
## 
## ─ Packages ───────────────────────────────────────────────────────────────────
##  package                * version   date (UTC) lib source
##  abind                    1.4-5     2016-07-21 [1] CRAN (R 4.3.0)
##  AnnotationDbi            1.62.2    2023-07-02 [1] Bioconductor
##  AnnotationHub            3.8.0     2023-05-08 [1] Bioconductor
##  ash                      1.0-15    2015-09-01 [1] CRAN (R 4.3.0)
##  askpass                  1.2.0     2023-09-03 [1] CRAN (R 4.3.0)
##  backports                1.4.1     2021-12-13 [1] CRAN (R 4.3.0)
##  base64enc                0.1-3     2015-07-28 [1] CRAN (R 4.3.0)
##  beachmat                 2.16.0    2023-05-08 [1] Bioconductor
##  BH                       1.81.0-1  2023-01-22 [1] CRAN (R 4.3.0)
##  Biobase                  2.60.0    2023-05-08 [1] Bioconductor
##  BiocFileCache            2.8.0     2023-05-08 [1] Bioconductor
##  BiocGenerics             0.46.0    2023-06-04 [1] Bioconductor
##  BiocManager              1.30.22   2023-08-08 [1] CRAN (R 4.3.0)
##  BiocParallel             1.34.2    2023-05-28 [1] Bioconductor
##  BiocVersion              3.17.1    2022-12-20 [1] Bioconductor
##  Biostrings               2.68.1    2023-05-21 [1] Bioconductor
##  bit                      4.0.5     2022-11-15 [1] CRAN (R 4.3.0)
##  bit64                    4.0.5     2020-08-30 [1] CRAN (R 4.3.0)
##  bitops                   1.0-7     2021-04-24 [1] CRAN (R 4.3.0)
##  blob                     1.2.4     2023-03-17 [1] CRAN (R 4.3.0)
##  boot                     1.3-28.1  2022-11-22 [1] CRAN (R 4.3.1)
##  brio                     1.1.3     2021-11-30 [1] CRAN (R 4.3.0)
##  broom                    1.0.5     2023-06-09 [1] CRAN (R 4.3.0)
##  bslib                    0.5.1     2023-08-11 [1] CRAN (R 4.3.0)
##  cachem                   1.0.8     2023-05-01 [1] CRAN (R 4.3.0)
##  callr                    3.7.3     2022-11-02 [1] CRAN (R 4.3.0)
##  cellranger               1.1.0     2016-07-27 [1] CRAN (R 4.3.0)
##  cli                      3.6.1     2023-03-23 [1] CRAN (R 4.3.0)
##  clipr                    0.8.0     2022-02-22 [1] CRAN (R 4.3.0)
##  cluster                  2.1.4     2022-08-22 [1] CRAN (R 4.3.1)
##  codetools                0.2-19    2023-02-01 [1] CRAN (R 4.3.1)
##  colorspace               2.1-0     2023-01-23 [1] CRAN (R 4.3.0)
##  commonmark               1.9.0     2023-03-17 [1] CRAN (R 4.3.0)
##  conflicted               1.2.0     2023-02-01 [1] CRAN (R 4.3.0)
##  cpp11                    0.4.7     2023-12-02 [1] CRAN (R 4.3.1)
##  crayon                   1.5.2     2022-09-29 [1] CRAN (R 4.3.0)
##  crosstalk                1.2.0     2021-11-04 [1] CRAN (R 4.3.0)
##  curl                     5.0.2     2023-08-14 [1] CRAN (R 4.3.0)
##  data.table               1.14.8    2023-02-17 [1] CRAN (R 4.3.0)
##  DBI                      1.1.3     2022-06-18 [1] CRAN (R 4.3.0)
##  dbplyr                   2.3.4     2023-09-26 [1] CRAN (R 4.3.1)
##  DelayedArray             0.26.7    2023-07-30 [1] Bioconductor
##  DelayedMatrixStats       1.22.6    2023-09-03 [1] Bioconductor
##  deldir                   2.0-2     2023-11-23 [1] CRAN (R 4.3.1)
##  desc                     1.4.2     2022-09-08 [1] CRAN (R 4.3.0)
##  deSolve                  1.40      2023-11-27 [1] CRAN (R 4.3.1)
##  diffobj                  0.3.5     2021-10-05 [1] CRAN (R 4.3.0)
##  digest                   0.6.33    2023-07-07 [1] CRAN (R 4.3.0)
##  dixon                    0.0-8     2022-02-12 [1] CRAN (R 4.3.0)
##  dplyr                    1.1.4     2023-11-17 [1] CRAN (R 4.3.1)
##  dqrng                    0.3.1     2023-08-30 [1] CRAN (R 4.3.0)
##  DropletUtils             1.20.0    2023-05-08 [1] Bioconductor
##  DT                       0.29      2023-08-29 [1] CRAN (R 4.3.0)
##  dtplyr                   1.3.1     2023-03-22 [1] CRAN (R 4.3.0)
##  edgeR                    3.42.4    2023-06-04 [1] Bioconductor
##  ellipsis                 0.3.2     2021-04-29 [1] CRAN (R 4.3.0)
##  evaluate                 0.23      2023-11-01 [1] CRAN (R 4.3.1)
##  ExperimentHub            2.8.1     2023-07-16 [1] Bioconductor
##  fansi                    1.0.5     2023-10-08 [1] CRAN (R 4.3.1)
##  farver                   2.1.1     2022-07-06 [1] CRAN (R 4.3.0)
##  fastmap                  1.1.1     2023-02-24 [1] CRAN (R 4.3.0)
##  fda                      6.1.4     2023-05-23 [1] CRAN (R 4.3.0)
##  fds                      1.8       2018-10-31 [1] CRAN (R 4.3.0)
##  filelock                 1.0.2     2018-10-05 [1] CRAN (R 4.3.0)
##  FNN                      1.1.3.2   2023-03-20 [1] CRAN (R 4.3.0)
##  fontawesome              0.5.2     2023-08-19 [1] CRAN (R 4.3.0)
##  forcats                  1.0.0     2023-01-29 [1] CRAN (R 4.3.0)
##  formatR                  1.14      2023-01-17 [1] CRAN (R 4.3.0)
##  fs                       1.6.3     2023-07-20 [1] CRAN (R 4.3.0)
##  furrr                    0.3.1     2022-08-15 [1] CRAN (R 4.3.0)
##  futile.logger            1.4.3     2016-07-10 [1] CRAN (R 4.3.0)
##  futile.options           1.0.1     2018-04-20 [1] CRAN (R 4.3.0)
##  future                   1.33.0    2023-07-01 [1] CRAN (R 4.3.0)
##  gamm4                    0.2-6     2020-04-03 [1] CRAN (R 4.3.0)
##  gargle                   1.5.2     2023-07-20 [1] CRAN (R 4.3.0)
##  generics                 0.1.3     2022-07-05 [1] CRAN (R 4.3.0)
##  GenomeInfoDb             1.36.3    2023-09-10 [1] Bioconductor
##  GenomeInfoDbData         1.2.10    2023-08-04 [1] Bioconductor
##  GenomicRanges            1.52.0    2023-05-08 [1] Bioconductor
##  ggplot2                  3.4.4     2023-10-12 [1] CRAN (R 4.3.1)
##  globals                  0.16.2    2022-11-21 [1] CRAN (R 4.3.0)
##  glue                     1.6.2     2022-02-24 [1] CRAN (R 4.3.0)
##  goftest                  1.2-3     2021-10-07 [1] CRAN (R 4.3.0)
##  googledrive              2.1.1     2023-06-11 [1] CRAN (R 4.3.0)
##  googlesheets4            1.1.1     2023-06-11 [1] CRAN (R 4.3.0)
##  grpreg                   3.4.0     2021-07-26 [1] CRAN (R 4.3.0)
##  gtable                   0.3.4     2023-08-21 [1] CRAN (R 4.3.0)
##  haven                    2.5.3     2023-06-30 [1] CRAN (R 4.3.0)
##  HDF5Array                1.28.1    2023-05-08 [1] Bioconductor
##  hdrcde                   3.4       2021-01-18 [1] CRAN (R 4.3.0)
##  highr                    0.10      2022-12-22 [1] CRAN (R 4.3.0)
##  hms                      1.1.3     2023-03-21 [1] CRAN (R 4.3.0)
##  htmltools                0.5.6     2023-08-10 [1] CRAN (R 4.3.0)
##  htmlwidgets              1.6.2     2023-03-17 [1] CRAN (R 4.3.0)
##  httpuv                   1.6.11    2023-05-11 [1] CRAN (R 4.3.0)
##  httr                     1.4.7     2023-08-15 [1] CRAN (R 4.3.0)
##  ids                      1.0.1     2017-05-31 [1] CRAN (R 4.3.0)
##  interactiveDisplayBase   1.38.0    2023-05-08 [1] Bioconductor
##  IRanges                  2.34.1    2023-07-02 [1] Bioconductor
##  isoband                  0.2.7     2022-12-20 [1] CRAN (R 4.3.0)
##  jquerylib                0.1.4     2021-04-26 [1] CRAN (R 4.3.0)
##  jsonlite                 1.8.8     2023-12-04 [1] CRAN (R 4.3.1)
##  KEGGREST                 1.40.0    2023-05-08 [1] Bioconductor
##  kernlab                  0.9-32    2023-01-31 [1] CRAN (R 4.3.0)
##  KernSmooth               2.23-22   2023-07-10 [1] CRAN (R 4.3.0)
##  knitr                    1.44      2023-09-11 [1] CRAN (R 4.3.0)
##  ks                       1.14.1    2023-08-10 [1] CRAN (R 4.3.0)
##  labeling                 0.4.3     2023-08-29 [1] CRAN (R 4.3.0)
##  lambda.r                 1.2.4     2019-09-18 [1] CRAN (R 4.3.0)
##  later                    1.3.1     2023-05-02 [1] CRAN (R 4.3.0)
##  lattice                  0.21-8    2023-04-05 [1] CRAN (R 4.3.1)
##  lazyeval                 0.2.2     2019-03-15 [1] CRAN (R 4.3.0)
##  lifecycle                1.0.4     2023-11-07 [1] CRAN (R 4.3.1)
##  limma                    3.56.2    2023-06-04 [1] Bioconductor
##  listenv                  0.9.0     2022-12-16 [1] CRAN (R 4.3.0)
##  lme4                     1.1-35.1  2023-11-05 [1] CRAN (R 4.3.1)
##  locfit                   1.5-9.8   2023-06-11 [1] CRAN (R 4.3.0)
##  lubridate                1.9.3     2023-09-27 [1] CRAN (R 4.3.1)
##  magic                    1.6-1     2022-11-16 [1] CRAN (R 4.3.0)
##  magick                   2.8.0     2023-09-25 [1] CRAN (R 4.3.1)
##  magrittr                 2.0.3     2022-03-30 [1] CRAN (R 4.3.0)
##  MASS                     7.3-60    2023-05-04 [1] CRAN (R 4.3.1)
##  Matrix                   1.6-1.1   2023-09-18 [1] CRAN (R 4.3.1)
##  MatrixGenerics           1.12.3    2023-07-30 [1] Bioconductor
##  matrixStats              1.0.0     2023-06-02 [1] CRAN (R 4.3.0)
##  mclust                   6.0.1     2023-11-15 [1] CRAN (R 4.3.1)
##  memoise                  2.0.1     2021-11-26 [1] CRAN (R 4.3.0)
##  mgcv                     1.9-0     2023-07-11 [1] CRAN (R 4.3.0)
##  mime                     0.12      2021-09-28 [1] CRAN (R 4.3.0)
##  minqa                    1.2.6     2023-09-11 [1] CRAN (R 4.3.0)
##  misc3d                   0.9-1     2021-10-07 [1] CRAN (R 4.3.0)
##  modelr                   0.1.11    2023-03-22 [1] CRAN (R 4.3.0)
##  multicool                1.0.0     2023-12-02 [1] CRAN (R 4.3.1)
##  munsell                  0.5.0     2018-06-12 [1] CRAN (R 4.3.0)
##  mvtnorm                  1.2-4     2023-11-27 [1] CRAN (R 4.3.1)
##  mxfda                    0.1.0     2023-12-07 [1] local
##  nlme                     3.1-163   2023-08-09 [1] CRAN (R 4.3.0)
##  nloptr                   2.0.3     2022-05-26 [1] CRAN (R 4.3.0)
##  openssl                  2.1.1     2023-09-25 [1] CRAN (R 4.3.1)
##  parallelly               1.36.0    2023-05-26 [1] CRAN (R 4.3.0)
##  pbmcapply                1.5.1     2022-04-28 [1] CRAN (R 4.3.0)
##  pbs                      1.1       2013-06-08 [1] CRAN (R 4.3.0)
##  pcaPP                    2.0-3     2022-10-24 [1] CRAN (R 4.3.0)
##  pillar                   1.9.0     2023-03-22 [1] CRAN (R 4.3.0)
##  pkgbuild                 1.4.2     2023-06-26 [1] CRAN (R 4.3.0)
##  pkgconfig                2.0.3     2019-09-22 [1] CRAN (R 4.3.0)
##  pkgload                  1.3.3     2023-09-22 [1] CRAN (R 4.3.1)
##  plogr                    0.2.0     2018-03-25 [1] CRAN (R 4.3.0)
##  plot3D                   1.4       2021-05-22 [1] CRAN (R 4.3.0)
##  plyr                     1.8.9     2023-10-02 [1] CRAN (R 4.3.1)
##  png                      0.1-8     2022-11-29 [1] CRAN (R 4.3.0)
##  polyclip                 1.10-6    2023-09-27 [1] CRAN (R 4.3.1)
##  pracma                   2.4.4     2023-11-10 [1] CRAN (R 4.3.1)
##  praise                   1.0.0     2015-08-11 [1] CRAN (R 4.3.0)
##  prettyunits              1.2.0     2023-09-24 [1] CRAN (R 4.3.1)
##  processx                 3.8.2     2023-06-30 [1] CRAN (R 4.3.0)
##  progress                 1.2.2     2019-05-16 [1] CRAN (R 4.3.0)
##  promises                 1.2.1     2023-08-10 [1] CRAN (R 4.3.0)
##  ps                       1.7.5     2023-04-18 [1] CRAN (R 4.3.0)
##  purrr                    1.0.2     2023-08-10 [1] CRAN (R 4.3.0)
##  R.methodsS3              1.8.2     2022-06-13 [1] CRAN (R 4.3.0)
##  R.oo                     1.25.0    2022-06-12 [1] CRAN (R 4.3.0)
##  R.utils                  2.12.2    2022-11-11 [1] CRAN (R 4.3.0)
##  R6                       2.5.1     2021-08-19 [1] CRAN (R 4.3.0)
##  ragg                     1.2.5     2023-01-12 [1] CRAN (R 4.3.0)
##  rainbow                  3.7       2022-10-09 [1] CRAN (R 4.3.0)
##  rappdirs                 0.3.3     2021-01-31 [1] CRAN (R 4.3.0)
##  RColorBrewer             1.1-3     2022-04-03 [1] CRAN (R 4.3.0)
##  Rcpp                     1.0.11    2023-07-06 [1] CRAN (R 4.3.0)
##  RcppEigen                0.3.3.9.4 2023-11-02 [1] CRAN (R 4.3.1)
##  RCurl                    1.98-1.13 2023-11-02 [1] CRAN (R 4.3.1)
##  readr                    2.1.4     2023-02-10 [1] CRAN (R 4.3.0)
##  readxl                   1.4.3     2023-07-06 [1] CRAN (R 4.3.0)
##  refund                   0.1-33    2023-11-14 [1] CRAN (R 4.3.1)
##  rematch                  2.0.0     2023-08-30 [1] CRAN (R 4.3.0)
##  rematch2                 2.1.2     2020-05-01 [1] CRAN (R 4.3.0)
##  reprex                   2.0.2     2022-08-17 [1] CRAN (R 4.3.0)
##  reshape2                 1.4.4     2020-04-09 [1] CRAN (R 4.3.0)
##  rhdf5                    2.44.0    2023-05-08 [1] Bioconductor
##  rhdf5filters             1.12.1    2023-05-08 [1] Bioconductor
##  Rhdf5lib                 1.22.1    2023-09-10 [1] Bioconductor
##  rjson                    0.2.21    2022-01-09 [1] CRAN (R 4.3.0)
##  rlang                    1.1.2     2023-11-04 [1] CRAN (R 4.3.1)
##  RLRsim                   3.1-8     2022-03-16 [1] CRAN (R 4.3.0)
##  rmarkdown                2.25      2023-09-18 [1] CRAN (R 4.3.1)
##  rpart                    4.1.19    2022-10-21 [1] CRAN (R 4.3.1)
##  rprojroot                2.0.4     2023-11-05 [1] CRAN (R 4.3.1)
##  RSQLite                  2.3.1     2023-04-03 [1] CRAN (R 4.3.0)
##  rstudioapi               0.15.0    2023-07-07 [1] CRAN (R 4.3.0)
##  rvest                    1.0.3     2022-08-19 [1] CRAN (R 4.3.0)
##  S4Arrays                 1.0.6     2023-09-10 [1] Bioconductor
##  S4Vectors                0.38.1    2023-05-08 [1] Bioconductor
##  sass                     0.4.7     2023-07-15 [1] CRAN (R 4.3.0)
##  scales                   1.3.0     2023-11-28 [1] CRAN (R 4.3.1)
##  scuttle                  1.9.4     2023-01-23 [1] Bioconductor
##  selectr                  0.4-2     2019-11-20 [1] CRAN (R 4.3.0)
##  shiny                    1.7.5     2023-08-12 [1] CRAN (R 4.3.0)
##  SingleCellExperiment     1.22.0    2023-05-08 [1] Bioconductor
##  sitmo                    2.0.2     2021-10-13 [1] CRAN (R 4.3.0)
##  snow                     0.4-4     2021-10-27 [1] CRAN (R 4.3.0)
##  sourcetools              0.1.7-1   2023-02-01 [1] CRAN (R 4.3.0)
##  sp                       2.0-0     2023-06-22 [1] CRAN (R 4.3.0)
##  sparseMatrixStats        1.12.2    2023-07-02 [1] Bioconductor
##  SpatialExperiment        1.10.0    2023-05-08 [1] Bioconductor
##  spatialTIME              1.3.4-2   2023-12-19 [1] CRAN (R 4.3.1)
##  spatstat                 3.0-7     2023-10-30 [1] CRAN (R 4.3.1)
##  spatstat.data            3.0-3     2023-10-24 [1] CRAN (R 4.3.1)
##  spatstat.explore         3.2-5     2023-10-22 [1] CRAN (R 4.3.1)
##  spatstat.geom            3.2-7     2023-10-20 [1] CRAN (R 4.3.1)
##  spatstat.linnet          3.1-3     2023-10-28 [1] CRAN (R 4.3.1)
##  spatstat.model           3.2-8     2023-10-23 [1] CRAN (R 4.3.1)
##  spatstat.random          3.2-2     2023-11-29 [1] CRAN (R 4.3.1)
##  spatstat.sparse          3.0-3     2023-10-24 [1] CRAN (R 4.3.1)
##  spatstat.utils           3.0-4     2023-10-24 [1] CRAN (R 4.3.1)
##  splancs                  2.01-44   2023-08-21 [1] CRAN (R 4.3.0)
##  stringi                  1.8.2     2023-11-23 [1] CRAN (R 4.3.1)
##  stringr                  1.5.1     2023-11-14 [1] CRAN (R 4.3.1)
##  SummarizedExperiment     1.30.2    2023-06-11 [1] Bioconductor
##  sys                      3.4.2     2023-05-23 [1] CRAN (R 4.3.0)
##  systemfonts              1.0.4     2022-02-11 [1] CRAN (R 4.3.0)
##  tensor                   1.5       2012-05-05 [1] CRAN (R 4.3.0)
##  testthat                 3.2.1     2023-12-02 [1] CRAN (R 4.3.1)
##  textshaping              0.3.6     2021-10-13 [1] CRAN (R 4.3.0)
##  tibble                   3.2.1     2023-03-20 [1] CRAN (R 4.3.0)
##  tidyr                    1.3.0     2023-01-24 [1] CRAN (R 4.3.0)
##  tidyselect               1.2.0     2022-10-10 [1] CRAN (R 4.3.0)
##  tidyverse                2.0.0     2023-02-22 [1] CRAN (R 4.3.0)
##  timechange               0.2.0     2023-01-11 [1] CRAN (R 4.3.0)
##  tinytex                  0.46      2023-08-09 [1] CRAN (R 4.3.0)
##  tzdb                     0.4.0     2023-05-12 [1] CRAN (R 4.3.0)
##  utf8                     1.2.4     2023-10-22 [1] CRAN (R 4.3.1)
##  uuid                     1.1-1     2023-08-17 [1] CRAN (R 4.3.0)
##  vctrs                    0.6.5     2023-12-01 [1] CRAN (R 4.3.1)
##  VectraPolarisData        1.4.0     2023-04-27 [1] Bioconductor
##  viridisLite              0.4.2     2023-05-02 [1] CRAN (R 4.3.0)
##  vroom                    1.6.3     2023-04-28 [1] CRAN (R 4.3.0)
##  waldo                    0.5.2     2023-11-02 [1] CRAN (R 4.3.1)
##  withr                    2.5.2     2023-10-30 [1] CRAN (R 4.3.1)
##  xfun                     0.40      2023-08-09 [1] CRAN (R 4.3.0)
##  xml2                     1.3.5     2023-07-06 [1] CRAN (R 4.3.0)
##  xtable                   1.8-4     2019-04-21 [1] CRAN (R 4.3.0)
##  XVector                  0.40.0    2023-05-08 [1] Bioconductor
##  yaml                     2.3.7     2023-01-23 [1] CRAN (R 4.3.0)
##  zlibbioc                 1.46.0    2023-05-08 [1] Bioconductor
## 
##  [1] /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library
## 
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